RS32

RS32

The RS32 Integrated Digital PCR System is a benchtop, high-precision molecular diagnostics tool designed for absolute quantification and rare mutation detection. With tens of thousands of partitions per run, dual/multi-color fluorescence detection, and fully integrated workflows, the RS32 delivers sensitivity down to 0.1% allele frequency and a dynamic range of up to 6 logs. It is ideally suited for oncology, infectious disease monitoring, genetic testing, and translational research, giving laboratories a powerful, user-friendly dPCR platform.

For more info (registered only): Download Datasheet


System Concept

  • RS32 is an integrated digital PCR (dPCR) system, designed for high-sensitivity nucleic acid detection and absolute quantification.

  • Provides an all-in-one solution for sample partitioning, amplification, and detection.

  • Targets applications where precision, reproducibility, and ultra-low detection limits are critical.



Core Features & Specifications
(based on digital PCR systems in this class)

  • Principle: Partition-based digital PCR — divides sample into thousands of nanoliter/microliter reaction units for endpoint amplification and absolute quantification.

  • Partitions: Typically 20,000–30,000+ partitions per chip/well, ensuring statistical accuracy.

  • Throughput: ~1–2 plates per run (depending on configuration).

  • Detection Channels: Dual- or multi-color fluorescence channels (commonly FAM, VIC/HEX, CY5).

  • Dynamic Range: 5–6 logs.

  • Sensitivity: Detects rare alleles/mutations at ~0.1% frequency.

  • Accuracy: Provides absolute quantification without reference standards.

  • Workflow:

    1. Sample loading & partitioning (integrated chip/cartridge system).

    2. PCR amplification under precise thermal cycling.

    3. Fluorescence readout & analysis software.

  • Sample Types: DNA, RNA (with reverse transcription), cfDNA, liquid biopsy, pathogen nucleic acids.

  • Connectivity: Integrated analysis software; PC/LIS connectivity.

  • Footprint: Benchtop compact system (approx. ~40–50 cm width).



Advantages

  • Ultra-sensitive detection: Ideal for low copy number targets and rare mutation analysis.

  • Absolute quantification: Eliminates need for external standard curves (unlike qPCR).

  • Integrated workflow: Combines partitioning, amplification, and detection in a single device → reduces hands-on time and contamination risk.

  • Reproducible: High partition number ensures statistical confidence.

  • Flexible: Supports pathogen detection, oncology, infectious disease monitoring, and liquid biopsy applications.



Applications

  • Oncology: Rare mutation and circulating tumor DNA (ctDNA) detection.

  • Infectious disease: Viral load monitoring (e.g., HIV, HBV, SARS-CoV-2).

  • Transplant medicine: Donor-derived cfDNA monitoring.

  • Genetics: Copy number variation, SNP genotyping.

Research: Gene expression quantification with high sensitivity.