LiveCodim

LiveCodim transforms an existing inverted microscope into a flexible widefield–confocal–super-resolution system that delivers ~90 nm lateral resolution while minimizing phototoxicity and photobleaching, making it well-suited for live-cell imaging. Using conical diffraction from a biaxial crystal to generate structured illumination patterns, it reconstructs super-resolved images across up to four laser channels, with confocal-like depth reach (to ~500 μm). The GPU-accelerated software streamlines acquisition and processing, outputs OME-TIFF, and integrates smoothly with ImageJ/Imaris. Because it’s achromatic, modular, and microscope-agnostic, LiveCodim is a practical way to add high-performance super-resolution to standard confocal workflows—without changing sample prep.

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What it is

• 3-in-1 imaging platform that upgrades a standard widefield microscope to widefield, confocal, and super-resolution modes.
  

• Based on conical diffraction with a biaxial crystal to create structured illumination; scans patterns in three orientations for super-resolved reconstructions.
  

Key capabilities

• Gentle super-resolution: up to ~90 nm lateral (at 488 nm) with low phototoxicity and little-to-no photobleaching.
  

• Depth penetration comparable to confocal; imaging up to ~500 μm (with long-WD objectives).
  

• Multicolor: up to 4 laser lines (405/488/561/640 nm; customizable).
  

• Switch modes with one click; no change in sample prep vs. confocal.
  

Compatibility & integration

• Achromatic & modular (400–1000 nm); fits any major inverted microscope with left/right side port (e.g., Nikon Ti, Zeiss AxioObserver, Evident IX83, Leica DMi…).
  

• Works with incubators/CO₂ fittings, standard LED widefield illumination, filter turrets/cubes, high-NA oil objectives (60×/63×, NA 1.40–1.49), motorized Z and XY.
  

• Outputs OME-TIFF; TIFF for images.
  

Software & workflow

• Intuitive UI for acquire/process/visualize; GPU-accelerated reconstruction; adaptive SR processing.
  

• FAIR data; direct export to ImageJ & Imaris; communicates with open-source tools.
  

Performance & specs

• Lateral resolution: up to ~90 nm (488 nm, high-NA oil).
  

• Axial resolution: up to ~500 nm (488 nm, high-NA oil).
  

• Max scanned FOV: 60 × 60 μm (60× objective).
  

• Acquisition speed: ~2 s per 10 × 10 μm area.
  

• Camera: sCMOS (≈96% QE).
  

• Operation: point scanning with structured PSF; variable pinhole.
  

• Footprint: ~52 × 48 × 14.5 cm, ~20 kg.
  

Applications

• Cell biology, cytoskeleton dynamics, cell cycle, cancer biology, microbiology, neurobiology, plant biology—and broadly “your sample”.
  

Why it’s different

• True optical super-resolution (not just deconvolution) with low-intensity illumination window → preserves live cells.
  

• Confocal-like geometry + structured illumination → SR detail with deeper scanning.
  

No special dyes/probes beyond standard confocal labeling; no exotic sample prep.